Humanized BAFF transgenic mice develop autoimmune manifestations

Abstract The B cell activating factor(BAFF) is a survival factor that supports autoreactive cells’ and prevents their depletion. Excessive BAFF can increase cells, driving autoimmunity. commonly overexpressed in Systemic Lupus Erythematosus(SLE) strongly involved the pathogenesis of disease. Mice with deficiency results lack mature whereas mice overproduce have high numbers cells antibodies, including autoantibodies, develop an autoimmune disease similar to SLE humans. has been shown be pivotal target diseases. In this study, we report generation two transgenic mouse strains overexpression human (B6-hBAFF, T036791 T036794), which could used study anti-BAFF therapies. Overexpression led significantly increased IgG, IgA, IgM levels since 6 weeks age compared wild-type mice, continued gradually as aged. These secreted level anti-DNA along ratio cells/CD45 +in spleen lymph node indicating overreactive cells. Proteinuria started at protein/creatinine ratio. This was accompanied by infiltration inflammatory leukocytes kidney 15 age, suggesting local inflammation. Taken together, humanized manifestations enhanced providing valuable tool supporting preclinical efficacy studies drugs targeting for development